| CSA Matthews Predator G | Charlotte Soccer Academy |
| FC Alliance ECNL U15G | FC Alliance (TN) |
| HFC Red G | Highland Football Club (HFC) |
| LFC 03 Red | Lexington Football Club |
| Ohio Extreme SC Lima Girls 2003 | Ohio Extreme SC |
| TSC 03G Premier | Tennessee Soccer Club (TN) |
| UFA Forsyth 03G Red | United Futbol Academy (GA) |
| Warren County SA Wcsa 2003 Elite | Warren County SA |
| WCSA G03 Premier | Warren County SA |
| WVFC 2003 Girls - Black | West Virginia Football Club |
| BSC 02G | Bluegrass SC |
| Butler United 02 Extreme | Ohio |
| CMSC Green G | Carolina Mountains Soccer Club |
| CSC 02/03 Girls | Clarksville Soccer Club (TN) |
| FC Alliance 02G Red | FC Alliance (TN) |
| FC Alliance ECNL U16 | FC Alliance (TN) |
| FCA Armada 02 Premier | FC Academy Nashville |
| Galaxy 2002 Black | Galaxy Soccer Club |
| HFC 02G Elite Wolves | Hoosier Futbol Club |
| HFC Red G | Highland Football Club (HFC) |
| LFC 02 Girls White | Lexington Football Club |
| WCSA 02G PREMIER | Warren County SA |
| WVFC 2002 Girls - Black | West Virginia Football Club |
| Arsenal '01 | FC Tippecanoe |
| Crew SC Elite 2001 | Columbus Crew SC |
| Derby City Rovers 01 Girls | Derby City Rovers |
| Eastern Elite Soccer Academy 2001 Girls | Eastern Elite Soccer Accademy |
| FC Alliance 01 | FC Alliance (TN) |
| FC Alliance 01G Red | FC Alliance (TN) |
| FC Dallas TRI 01/02 Girls McLeod | FC Dallas TRI |
| GSA U16 girls | Greeneville Soccer Association |
| HFC Red G | Highland Football Club (HFC) |
| Impact SC 01G Elite | Cherokee Impact |
| Lexington FC Lfc 01 Red | Lexington Football Club |
| Metro FC 01G Rapids | Metro FC (OH) |
| MSC Lady Strikers 01 Elite | Murfreesboro Soccer Club |
| MSC Lady Strikers 01 Premier | Murfreesboro Soccer Club |
| Nashville FC Youth 2001 Girls | Nashville FC (TN) |
| NUSA 01G Maroon | Nashville United Soccer Academy |
| Rossoneri 01 Black | Derby City Rossoneri |
| Tennessee Soccer Club 01 Showcase | Tennessee Soccer Club (TN) |
| CMSC GREEEN G | Carolina Mountains Soccer Club |
| CVYSA Blast Blue G | Catawba Valley YSA |
| Emerald Force 00 Valencia | Emerald Force Soccer Club |
| FC Dallas TRI 00/01G Premier | FC Dallas TRI |
| Girls U18 Orange | FC614 |
| HFC Red G | Highland Football Club (HFC) |
| Lady Legacy | Lakeway Soccer Club |
| MFFC 00G Premier | McMinn Force Futbol Club |
| USA-NORTH 00G | USA North |
| WCSA 00G PREMIER | Warren County SA |
| Butler United 99 Bullets | Ohio |
| FC Alliance 99G ECNL Composite | FC Alliance (TN) |
| FC Alliance ECNL U18/U19 | FC Alliance (TN) |
| HFC Red II G | Highland Football Club (HFC) |
| Lookout FC | Tennessee |
| LYSA Force G | Lenoir Youth Soccer |
| MSC Lady Strikers 99 Premier | Murfreesboro Soccer Club |
| SWVA RUSH 99G NERO | SWVA Rush |