| FC Alliance 04 ECNL | FC Alliance (TN) | TN |
| LFC 04G White | Lexington FC | KY |
| LNSC Eclipse Blue G | Lake Norman Soccer Club (LNSC) | NC |
| UFA Forsyth 04G Premier | United Futbol Academy (GA) | GA |
| Eastern Elite 05 Girls | Eastern Elite Soccer Accademy | KY |
| FC Alliance 05 ECNL | FC Alliance (TN) | TN |
| Metro FC 04G Rapids | Metro FC (OH) | OH |
| MFCY 05G Fury | Nashville FC (TN) | TN |
| 04 Usa North Challenge FC Girls | USA North | TN |
| Cleveland FC Lady Flames GU14 | Greater Cleveland Soccer Assn | TN |
| CVYSA BLAST BLUE G | Catawba Valley YSA | NC |
| FC Alliance 05G TSL | FC Alliance (TN) | TN |
| HFC Red G | Highland Football Club (HFC) | NC |
| WCSA 04G Premier | Warren County SA | OH |
| Knoxville FC Crush Premier 03G | Knoxville Football Club (TN) | TN |
| MSC 03 Lady Strikers | Murfreesboro Soccer Club | TN |
| Tri-Cities United 03/04 Girls Premier | Tri-Cities United Soccer Club | TN |
| UFA Forsyth 03G Red | United Futbol Academy (GA) | GA |
| WCSA G03 Premier | Warren County SA | OH |
| Emerald Force 03 Veracruz | Emerald Force Soccer Club | TN |
| LFC 03G Red | Lexington FC | KY |
| NUSA U16 GIRLS MAROON | Nashville United Soccer Acd (NUSA) | TN |
| Sporting Columbus 2003 | Sporting Columbus | OH |
| Tennessee Alliance 03 Girls | Tennessee Alliance FC | TN |
| FC ALLIANCE 02G ECNL | FC Alliance (TN) | TN |
| FC Alliance 03G ECNL | FC Alliance (TN) | TN |
| HFC Red G | Highland Football Club (HFC) | NC |
| TFA G02 Premier | Total Futbol Academy (OH) | OH |
| Warren County WC G02 Elite | Warren County SA | OH |
| WVFC 02G Black | West Virginia Football Club | WV |
| CSC 02 Girls | Clarksville Soccer Club (TN) | TN |
| FC Alliance 02G Red | FC Alliance (TN) | TN |
| Hoosier FC 2002 Girls Elite Wolves - Black | Hoosier Futbol Club | IN |
| Tennessee 02G Premier | Tennessee Soccer Club (TN) | TN |
| Butler United 02 Extreme | Butler United Soccer Club | OH |
| MFCY 02G Heat | Nashville FC (TN) | TN |
| NSA Belles 17 | Northwest Soccer Academy | GA |
| WCSA 02G PREMIER | Warren County SA | OH |
| BSC 02G | Bluegrass SC | KY |
| Club Ohio 02G Green 2 | Club Ohio Soccer | OH |
| CMSC Green G | Carolina Mountains Soccer Club | NC |
| USA North Girls 02/03 Premier | USA North | TN |
| Darlington School Soccer Academy U19 | Darlington School Soccer Academy | GA |
| FC Alliance 00/01 ECNL | FC Alliance (TN) | TN |
| FC Alliance 01 | FC Alliance (TN) | TN |
| Impact SC 01/02G Elite | Cherokee Impact | GA |
| TFA ELITE 00 | Total Futbol Academy (OH) | OH |
| WCSA 01G Elite | Warren County SA | OH |
| 01 CSA Palmetto King | CSA Palmetto United FC | SC |
| Eastern Elite Soccer Academy 2001 Girls | Eastern Elite Soccer Accademy | KY |
| Hoosier FC 2000 Elite Wolves | Hoosier Futbol Club | IN |
| NFC 01G | Nashville FC (TN) | TN |
| Tri-Cities United 01/02 Girls Premier | Tri-Cities United Soccer Club | TN |
| 01 HFC Red G | Highland Football Club (HFC) | NC |
| CVYSA Blast Blue G | Catawba Valley YSA | NC |
| GSA Lady Galaxy 01 | Greeneville Soccer Association | TN |
| Knoxville FC Crush Premier 00G | Knoxville Football Club (TN) | TN |
| KSA 00/01 Girls | Knoxville Soccer Academy | TN |
| TSC 2000 Girls Showcase | Tennessee Soccer Club (TN) | TN |
| Cleveland FC Lady Flames | Greater Cleveland Soccer Assn | TN |
| FC614 Girls Orange | FC614 | OH |
| HFC Red II G | Highland Football Club (HFC) | NC |
| Lookout FC 00 | Lookout FC | TN |
| Metro FC 01G Rapids | Metro FC (OH) | OH |
| MSC 01 Lady Strikers Premier | Murfreesboro Soccer Club | TN |