| CHSC 15U 03G (CHSC Elite) | Christiansburg Soccer Club | VA |
| FC Alliance ECNL U15g | FC Alliance (TN) | TN |
| Gtfc Impact 03 Premier | GTFC Impact | OH |
| Gtfc Impact G04 Premier | GTFC Impact | OH |
| LFC 03 White | Lexington Football Club | KY |
| United White 03 | Cincinnati West Soccer Club | OH |
| C4lfc Lakeland FC 03 Boys Black | Lakeland Futbol Club | FL |
| FC Alliance 03 Boys Npl | FC Alliance (TN) | TN |
| FC Alliance 04B ENPL | FC Alliance (TN) | TN |
| Lakeway Tornadoes | Lakeway Soccer Club | TN |
| PSA Predators | Premier Soccer Academy (TN) | TN |
| UCUSC 02 Cavalry | Upper Cumberland United Soccer Club | TN |
| Blaze 02 | Trussville United Soccer Club | AL |
| LFC u16 Girls Red | Lexington Football Club | KY |
| MFCY 02 The Heat | Nashville FC (TN) | TN |
| RCU Stars | River City United Soccer Club | AL |
| Darlington School Soccer Academy U16 | Darlington School Soccer Academy | GA |
| Eagle Express 01 Boys | Eagle Express Soccer Club | TN |
| FC Alliance 02 Boys ENPL | FC Alliance (TN) | TN |
| FC Alliance 02 Boys Red | FC Alliance (TN) | TN |
| Nashville FC 02 Boys Gold | Nashville FC (TN) | TN |
| NFC Navy 02 | Nashville FC (TN) | TN |
| All-In FC TN 01 Chippewas | All-In Futbol Club Tennessee | TN |
| Darlington School Soccer Academy U17 | Darlington School Soccer Academy | GA |
| Stones River FC 2001 Black | Stones River Futbol Club | TN |
| United FC - Furman 01B Elite | United FC - Furman | SC |
| FC Alliance 01 Boys RED | FC Alliance (TN) | TN |
| GPS NASA 01 BOYS MAROON | GPS NASA | SC |
| SCOR 01 Boys | Soccer Club of Oak Ridge (TN) | TN |
| United FC - Furman 01B Black | United FC - Furman | SC |
| CKSC Storm 00G Black | Central Kentucky Storm SC | KY |
| FC Alliance 01 | FC Alliance (TN) | TN |
| Lady Legacy | Lakeway Soccer Club | TN |
| MSC Lady Strikers 01 Premier | Murfreesboro Soccer Club | TN |
| Opelika Crush '00 Girls | Opelika Crush | AL |
| TSC 00G PDP | Technical Soccer Club | SC |
| FC Alliance 00B Red | FC Alliance (TN) | TN |
| Nationals Macomb 00B Black Select | Nationals Soccer Club | MI |
| SML Jets 00B | Smith Mountain Lake Jets | VA |
| Stones River FC 2000 | Stones River Futbol Club | TN |
| UCUSC Elite 99 Boys | Upper Cumberland United Soccer Club | TN |
| United FC - Furman 00B Black | United FC - Furman | SC |
| BUCKS F.C. | Wise County Youth Soccer | VA |
| BUSC 00B Hammers | Blount United Soccer Club | TN |
| Greeneville Galaxy Boys U18 | Greeneville Soccer Association | TN |
| PSA RIVER PLATE | Premier Soccer Academy (TN) | TN |
| Club Ohio Cincy Force | Club Ohio (OH) | OH |
| Crush '99 Lyon | Knoxville Football Club (TN) | TN |
| Darlington School Soccer Academy Girls U19 | Darlington School Soccer Academy | GA |
| FC Alliance 99G ECNL Composite | FC Alliance (TN) | TN |
| FC Alliance ECNL U18/U19 | FC Alliance (TN) | TN |
| MSC Lady Strikers 99 Premier | Murfreesboro Soccer Club | TN |
| Darlington School Soccer Academy U19 | Darlington School Soccer Academy | GA |
| FC Alliance 00B ENPL | FC Alliance (TN) | TN |
| FC Alliance 01 Boys ENPL | FC Alliance (TN) | TN |
| Lakeway Lightning | Lakeway Soccer Club | TN |
| MSC Strikers 99/00 Premier | Murfreesboro Soccer Club | TN |
| SCSA 00/99 Falcons Black | SCSA Falcons FC | TN |
| Auburn Thunder 99 Boys | Alabama | AL |
| BUSC Hammers 99B | Blount United Soccer Club | TN |
| Chattanooga FC 99/00 Elite Boys | Chattanooga FC Academy | TN |
| FC Alliance 99B Black | FC Alliance (TN) | TN |
| MUFC Boys 99 | McMinn United Football Club | TN |
| SSC 99B Select White | Summerville Soccer Club | SC |